منابع مشابه
Efficient liver gene transfer with foamy virus vectors
BACKGROUND Liver gene transfer offers hope for the correction of genetic and acquired disorders. Efficient gene transfer in large animals can be obtained with hydrodynamic gene transfer (HGT), a method that can achieve sufficient levels of gene delivery. MATERIAL AND METHODS To test the relative efficiency between plasmid versus foamy virus (FV) vector-based liver gene transfer efficiency, we...
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Highly active antiretroviral therapy (HAART) has vastly improved outcomes for patients infected with HIV, yet it is a lifelong regimen that is expensive and has significant side effects. Retroviral gene therapy is a promising alternative treatment for HIV/AIDS; however, inefficient gene delivery to hematopoietic stem cells (HSCs) has so far limited the efficacy of this approach. Foamy virus (FV...
متن کاملNonintegrating foamy virus vectors.
Foamy viruses (FVs), or spumaviruses, are integrating retroviruses that have been developed as vectors. Here we generated nonintegrating foamy virus (NIFV) vectors by introducing point mutations into the highly conserved DD35E catalytic core motif of the foamy virus integrase sequence. NIFV vectors produced high-titer stocks, transduced dividing cells, and did not integrate. Cells infected with...
متن کاملSequences in pol are required for transfer of human foamy virus-based vectors.
A series of vectors with heterologous genes was constructed from HSRV1, an infectious clone of human foamy virus (HFV), and transfected into baby hamster kidney cells to generate stably transfected vector cell lines. Two cis-acting sequences were required to achieve efficient rescue by helper virus. The first element was located at the 5' end upstream of position 1274 of the proviral DNA. Inter...
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The complexities of neurological diseases make it difficult to develop effective methods to treat them. In addition, it is difficult for the drugs used in neurological diseases to reach target cells at a sufficient concentration due to the blood-brain barrier, which may lead to side-effects in other organs, even though newly developed drugs are targeted directly to the origin of the diseases. N...
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ژورنال
عنوان ژورنال: Expert Opinion on Biological Therapy
سال: 2009
ISSN: 1471-2598,1744-7682
DOI: 10.1517/14712590903246388